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class b cpg oligodeoxynucleotide odn 2006  (InvivoGen)


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    InvivoGen class b cpg oligodeoxynucleotide odn 2006
    Class B Cpg Oligodeoxynucleotide Odn 2006, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpg+b+oligodeoxynucleotide+2006/ODN+2006/pm42026241-39-12-23
    Average 97 stars, based on 1475 article reviews
    class b cpg oligodeoxynucleotide odn 2006 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Platelet bound B cells and their role in SSc: Implications for disease subtypes and clinical outcomes.
    Article Snippet: Data acquisition were performed on a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). .. Cell culture and intracellular IL-10 by flow cytometry To evaluate intracellular production of IL-10, PBMCs were cultured in 96 well-plates (Thermo Fisher Scientific, Vienna, Austria) with complete RMPI-1640 medium and stimulated with 2.5 μg/mL of CPG-B oligodeoxynucleotide-2006 (ODN2006; InvivoGen, San Diego, CA, USA) and 2 μg/mL soluble CD40 ligand (sCD40L) (PeproTech EC, London, UK) during 48 hours in 5 % CO2 at 37◦C as previously described.24 For co-culture experiments, PBMCs without bound PLTs were isolated using a negative magnetic separation with biotinylated anti-CD41a (Miltenyi Biotec) and MagnisortTM positive selection beads (Thermo Fisher Scientific). .. PBMCs without bound PLTs from HD or SSc patients were cultured in the absence or presence of PLTs from HD or SSc patients at different PLT/PBMC ratios (50:1, 10:1, and 1:1) according to our previous studies.25 Prior to intracellular cytokine analysis, PBMCs were restimulated with 50 ng/ml phorbol myristate acetate (PMA, Sigma Aldrich, S. Louis, Missouri) and 500 ng/ml ionomycin (Sigma Aldrich) for four hours in 5 % CO2 at 37◦C.

    Article Title: Regulatory B cell frequency correlates with markers of HIV disease progression and attenuates anti-HIV CD8 + T cell function in vitro
    Article Snippet: To characterize TLR-activated Bregs, MACS-purified (Miltenyi Biotec, Auburn, CA, USA; according to the manufacturer's instructions) B cells from healthy controls were stained with antibodies against CD19-PE-CF594 (Beckman Coulter, Brea, CA, USA), CD24-PerCP-Cy5.5 (BD Biosciences, San Jose, CA, USA), and CD38-FITC (BD Biosciences). .. CD19 + CD24 hi CD38 hi (Bregs) and CD19 + CD24 lo CD38 lo cells (“non-Bregs”; mature B cells) were sorted (FACSAria; BD Biosciences) and cultured for 48 h in the presence of 10 μg/ml CpG-B oligodeoxynucleotide-2006, 2 μg/ml palmitoyl-3-cysteine-serine-lysine-4 (InvivoGen, San Diego, CA, USA), and 2 μg/ml CD40L (InvivoGen). .. During the final 5 h of incubation, the cultures were supplemented with PIB (PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:1000); InvivoGen and BD Biosciences).

    Flow Cytometry:

    Article Title: Platelet bound B cells and their role in SSc: Implications for disease subtypes and clinical outcomes.
    Article Snippet: Data acquisition were performed on a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). .. Cell culture and intracellular IL-10 by flow cytometry To evaluate intracellular production of IL-10, PBMCs were cultured in 96 well-plates (Thermo Fisher Scientific, Vienna, Austria) with complete RMPI-1640 medium and stimulated with 2.5 μg/mL of CPG-B oligodeoxynucleotide-2006 (ODN2006; InvivoGen, San Diego, CA, USA) and 2 μg/mL soluble CD40 ligand (sCD40L) (PeproTech EC, London, UK) during 48 hours in 5 % CO2 at 37◦C as previously described.24 For co-culture experiments, PBMCs without bound PLTs were isolated using a negative magnetic separation with biotinylated anti-CD41a (Miltenyi Biotec) and MagnisortTM positive selection beads (Thermo Fisher Scientific). .. PBMCs without bound PLTs from HD or SSc patients were cultured in the absence or presence of PLTs from HD or SSc patients at different PLT/PBMC ratios (50:1, 10:1, and 1:1) according to our previous studies.25 Prior to intracellular cytokine analysis, PBMCs were restimulated with 50 ng/ml phorbol myristate acetate (PMA, Sigma Aldrich, S. Louis, Missouri) and 500 ng/ml ionomycin (Sigma Aldrich) for four hours in 5 % CO2 at 37◦C.

    Isolation:

    Article Title: Platelet bound B cells and their role in SSc: Implications for disease subtypes and clinical outcomes.
    Article Snippet: Data acquisition were performed on a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). .. Cell culture and intracellular IL-10 by flow cytometry To evaluate intracellular production of IL-10, PBMCs were cultured in 96 well-plates (Thermo Fisher Scientific, Vienna, Austria) with complete RMPI-1640 medium and stimulated with 2.5 μg/mL of CPG-B oligodeoxynucleotide-2006 (ODN2006; InvivoGen, San Diego, CA, USA) and 2 μg/mL soluble CD40 ligand (sCD40L) (PeproTech EC, London, UK) during 48 hours in 5 % CO2 at 37◦C as previously described.24 For co-culture experiments, PBMCs without bound PLTs were isolated using a negative magnetic separation with biotinylated anti-CD41a (Miltenyi Biotec) and MagnisortTM positive selection beads (Thermo Fisher Scientific). .. PBMCs without bound PLTs from HD or SSc patients were cultured in the absence or presence of PLTs from HD or SSc patients at different PLT/PBMC ratios (50:1, 10:1, and 1:1) according to our previous studies.25 Prior to intracellular cytokine analysis, PBMCs were restimulated with 50 ng/ml phorbol myristate acetate (PMA, Sigma Aldrich, S. Louis, Missouri) and 500 ng/ml ionomycin (Sigma Aldrich) for four hours in 5 % CO2 at 37◦C.

    Selection:

    Article Title: Platelet bound B cells and their role in SSc: Implications for disease subtypes and clinical outcomes.
    Article Snippet: Data acquisition were performed on a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). .. Cell culture and intracellular IL-10 by flow cytometry To evaluate intracellular production of IL-10, PBMCs were cultured in 96 well-plates (Thermo Fisher Scientific, Vienna, Austria) with complete RMPI-1640 medium and stimulated with 2.5 μg/mL of CPG-B oligodeoxynucleotide-2006 (ODN2006; InvivoGen, San Diego, CA, USA) and 2 μg/mL soluble CD40 ligand (sCD40L) (PeproTech EC, London, UK) during 48 hours in 5 % CO2 at 37◦C as previously described.24 For co-culture experiments, PBMCs without bound PLTs were isolated using a negative magnetic separation with biotinylated anti-CD41a (Miltenyi Biotec) and MagnisortTM positive selection beads (Thermo Fisher Scientific). .. PBMCs without bound PLTs from HD or SSc patients were cultured in the absence or presence of PLTs from HD or SSc patients at different PLT/PBMC ratios (50:1, 10:1, and 1:1) according to our previous studies.25 Prior to intracellular cytokine analysis, PBMCs were restimulated with 50 ng/ml phorbol myristate acetate (PMA, Sigma Aldrich, S. Louis, Missouri) and 500 ng/ml ionomycin (Sigma Aldrich) for four hours in 5 % CO2 at 37◦C.



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    Cryopreserved and thawed PBMC were ml in flat-bottom plates in AIM V medium with 10% human AB serum for 18 hours with cultured at 5×10 6 / 3 µg/ml CpG-ODN type B-2006-G5 at 37 ° C, 5% CO 2 . PMA (50 ng/ml) and ionomycin (750 ng/ml) were added after 12 hours. Brefeldin and monensin (PTI)(3 µg/ml and 2 µM, respectively) were added after 14 hours. PBMCs were stained for viability (yellow amine dye) prior to surface epitope and intracellular cytokine staining. Cytokine quadrants were set based on isotype controls.

    Journal: medRxiv

    Article Title: IFNβ-1b treatment leads to changes in the B cell subset and cytokine secretion profile in patients with relapsing-remitting multiple sclerosis

    doi: 10.1101/2022.02.25.22270266

    Figure Lengend Snippet: Cryopreserved and thawed PBMC were ml in flat-bottom plates in AIM V medium with 10% human AB serum for 18 hours with cultured at 5×10 6 / 3 µg/ml CpG-ODN type B-2006-G5 at 37 ° C, 5% CO 2 . PMA (50 ng/ml) and ionomycin (750 ng/ml) were added after 12 hours. Brefeldin and monensin (PTI)(3 µg/ml and 2 µM, respectively) were added after 14 hours. PBMCs were stained for viability (yellow amine dye) prior to surface epitope and intracellular cytokine staining. Cytokine quadrants were set based on isotype controls.

    Article Snippet: Cryopreserved and thawed PBMC (for both patients and healthy controls) were cultured at 5×10 6 /ml in 12-well, flat-bottom plates in AIM V medium supplemented with 10% pooled human AB serum for 18 hours with 3 µg/ml CpG oligodeoxynucleotide type B-2006-G5 (InvivoGen, CA) at 37 ° C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Cell Culture, Staining